Rendered-Protein Hydrolysates as a Low-Cost Nitrogen Source for the Fungal Biotransformation of 5-Hydroxymethylfurfural

5-hydroxymethylfurfural (HMF) is a platform chemical that can be converted into wide range of high-value derivatives. Industrially, HMF-based derivatives are synthesized via catalysis. However, biocatalytic transformation has emerged as an attractive alternative. Significant advances have been made in the last years using isolated enzymes and whole-cell biocatalysts HMF biotransformation. Nonetheless, one major bottlenecks cost process, mainly due to microorganism growth substrate. In this work, biotransformation studies transform 2,5-di(hydroxymethyl)furan (DHMF) were carried out with fungus Fusarium striatum low-cost protein hydrolysates. The hydrolysates obtained from fines, unexploited material produced during rendering process meat industry waste residues. Given high content around 46%, hydrolysis was optimized two commercially available proteases, Alcalase 2.4 L Neutrase 0.8 L. maximum degree (DH) achieved 21.4% under optimal conditions 5% E/S ratio, pH 8, 55 °C, 24 h. On other hand, exhibited lower efficiency, therefore, recovery. After optimization response surface methodology (RSM), DH 7.2% variables set at 15% initial 40 10.5 Using these nitrogen source allowed higher sporulation and, use volume inoculum (three-fold), obtaining DHMF yield > 90%, 50% than when commercial peptones. presented allows animal co- by-products sources, which greatly impacts industrial feasibility